抗菌肽BuforinⅡ衍生物对金黄色葡萄球菌细胞膜作用机制的研究

doi:10.11843/j.issn.0366-6964.2013.07.018

Abstract

Abstract:

In this paper, the action mechanism of BF2-A/B, two analogues of antimicrobial peptide BuforinⅡ, on Staphylococcus aureus membrane had been researched. The results of FACScan analysis implied that BF2-A/B did not induce the influx of PI into the S. aureus cells. The rate of positive cells stained by fluorescent probe after BF2-B treatment was slightly higher than that of BF2-A. These electron micrographs showed that after 20 min of treatment by BF2-A, S. aureus cells still retained the plasma membrane integrality; however, BF2-B could cause some slight leakages of cellular cytoplasmic contents. The results of FACS analysis displayed that both the peptides could penetrate the cells, and BF2-B penetrated the cells more efficiently. The visualization of confocal microscopy proved that FITC-labeled BF2-A and BF2-B penetrated the bacterial cell membrane and accumulated in the cytoplasm of the cell immediately. The results of research demonstrated that BF2-A/B didn’t destroy the cell membrane of G⁺bacteria, and then exert the antimicrobial activity after influx into cytoplasm. BF2-B slightly disturbed cell membrane causing influx of PI and leakage of cytoplasmic contents during peptide crossing phospholipids bilayer. Meanwhile, the cell-penetrating efficiency of BF2-B was accordingly enhanced, which caused that BF2-B displayed more excellent antimicrobial activity to S. aureus.

CLC Number:

HAO Gang, LE Guo-wei, SHI Yong-hui. The Action Mechanism of Two Analogues of the Antimicrobial Peptide BuforinⅡ on Staphylococcus aureus Membrane[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1124-1130.

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The Action Mechanism of Two Analogues of the Antimicrobial Peptide BuforinⅡ on Staphylococcus aureus Membrane

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